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Chapters
- 00:00Title
- 00:49Introduction
- 01:21Intubation of Mice
- 03:39Setting Up an Intravenous Line
- 05:06Airway Resistance Measurements
- 06:22Bronchoalveolar Lavage
- 07:44Results of Airway Resistance Measurements
- 09:16Conclusion
Repeated measurements of rodent respiratory physiology and sampling of airway inflammatory cells are desirable, but generally not feasible. Here we describe a repeatable method for orally intubating mice that permits repeated measurements of airway hyperreactivity and sampling of airway inflammatory cells.
Tags
ReversibleNon-invasive MethodAirway Resistance MeasurementsBronchoalveolar Lavage Fluid SamplingMiceAirway HyperreactivityExperimental Asthma ModelsRepeated ProceduresAHR MeasurementsBAL Fluid SamplingProtocolsIntranasal ChallengeAllergenSham TreatmentAnesthetizedOrally IntubatedMechanically VentilatedDose Response CurvesRespiratory System Resistance (RRS)Acetylcholine (Ach) ChlorideAllergen-challenged AnimalsDifferential Enumeration Of Airway CellsRepeated Measurements Of AHR And BAL Fluid CollectionMaximal Airway HyperresponsivenessAirway EosinophiliaAllergen ChallengeLongitudinal Experimentation
