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JoVE Journal Neuroscience
Dissection et la culture de neurones de la moelle épinière commissurales embryonnaires
 
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Dissection et la culture de neurones de la moelle épinière commissurales embryonnaires

Article DOI: 10.3791/1773-v • 12:23 min • May 25th, 2010
May 25th, 2010 •
*1,2, *1, 1,3,4, 1,2,5,6,7
1Molecular Biology of Neural Development, Institut de Recherches Cliniques de Montréal, 2Division of Experimental Medicine and Program in Neuroengineering, McGill University, 3Program in Neuroengineering, McGill University, 4Montreal Neurological Institute, 5Department of Anatomy and Cell Biology, McGill University, 6Department of Biology, McGill University, 7Department of Medicine, Universite de Montreal - University of Montreal
* These authors contributed equally

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Cette vidéo montre une méthode de disséquer et de la culture des neurones commissuraux de la moelle épinière de rats E13 dorsale. Dissocié neurones commissuraux sont utiles pour étudier les mécanismes cellulaires et moléculaires de la croissance de l'axone et d'orientation.

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JoVE neurosciences numéro 39 le développement guidage axonal la moelle épinière la dissection la culture primaire Neuron Neurones commissurales
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