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1Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 2Broad Institute of Harvard and Massachusetts Institute of Technology, 3Division of Health Sciences and Technology,Massachusetts Institute of Technology, 4Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics,Harvard University , 5Department of Applied Mathematics,Harvard University , 6Department of Physics,Massachusetts Institute of Technology, 7Department of Systems Biology,Harvard Medical School, 8Department of Biology,Massachusetts Institute of Technology
Chapters
- 00:00Title
- 01:03Introduction
- 02:07Crosslinking, Digestion, Marking of DNA Ends, and Blunt-End Ligation
- 09:46Shearing and Size Selection
- 12:03Biotin Pull-Down and Paired End Sequencing
- 16:38Representative Hi-C Results
- 21:32Conclusion
De Hi-C methode maakt het mogelijk onpartijdige, genoom-brede identificatie van chromatine interacties (1). Hi-C paren nabijheid ligatie en massaal parallelle sequencing. De resulterende gegevens kunnen worden gebruikt om de genomische architectuur studeren op meerdere schalen: eerste resultaten geïdentificeerd functies zoals chromosoom gebieden, scheiding van open en gesloten chromatine, en chromatine structuur op de megabase schaal.
