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斑马鱼化工屏幕自动成像和分析的发展。
JoVE 신문
생물학
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JoVE 신문 생물학
Development of automated imaging and analysis for zebrafish chemical screens.
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斑马鱼化工屏幕自动成像和分析的发展。

DOI:
10.3791/1900-v

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10:49 min

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June 24, 2010

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, , , ,
1Pharmacology and Chemical Biology,University of Pittsburgh Drug Discovery Institute, 2Department of Microbiology and Molecular Genetics,University of Pittsburgh, 3Department of Pharmaceutical Sciences,University of Pittsburgh, 4Department of Chemistry,University of Pittsburgh

Chapters

  • 00:00Title
  • 00:53Introduction
  • 01:55Set Up Zebrafish Matings and BCl Treatment
  • 04:47Automated Imaging of 96-Well Plates and Analysis
  • 09:06Representative Results
  • 09:46Conclusion

Summary

자동 번역

자동 번역

我们报告的自动成像系统和分析在多孔板的转基因斑马鱼的胚胎的发展。这表明成纤维细胞生长因子记者的基因表达的能力来衡量的一个小分子，BCI，​​剂量依赖效应，并提供技术建立高通量的斑马鱼化工屏幕。

Tags

Automated ImagingAnalysisZebrafish Chemical ScreensImage-based High-content ScreeningFGF/RAS/MAPK PathwayZebrafish EmbryosIn Vivo High-content Chemical Screens96-well PlatesHigh Throughput ScreeningAutomated Image Acquisition And AnalysisTransgenic Reporter LineGreen Fluorescent Protein (GFP)Whole Organism ScreensAutomated Imaging And Analysis24-48 Hours Post Fertilization (hpf)Multiwell PlatesSmall MoleculeHyperactivates FGF Signaling

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