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Electron Cryotomography von Bakterienzellen
JoVE 신문
생물학
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JoVE 신문 생물학
Electron Cryotomography of Bacterial Cells
DOI:

14:23 min

May 06, 2010

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Chapters

  • 00:00Title
  • 00:39Introduction
  • 02:11Plunge Freezing Thin Bacterial Cells
  • 03:41High Pressure Freezing and Cryo Sectioning of Thicker Cells
  • 06:43Examination of the Cells Using the Cryo Light Microscope
  • 07:49Collecting Tilt Series Using the Cryo TEM
  • 09:08Tomogram Reconstruction and Database
  • 10:24Tomogram Segmentation
  • 10:51Movies and Animation
  • 11:22Representative Movies
  • 12:59Conclusion

Summary

자동 번역

Wir zeigen hier, wie Elektronen cryotomography (ECT) zu verwenden, um auf die Ultrastruktur von Bakterienzellen in nahezu native Staaten zu studieren, "makromolekularen" (~ 4 nm) Auflösung.

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