/

/

在哺乳动物细胞中的DNA双链断裂（DSB）的维修分析

JoVE 신문
생물학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생물학

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Please note that all translations are automatically generated. Click here for the English version.

在哺乳动物细胞中的DNA双链断裂（DSB）的维修分析

DOI:

•

13:10 min

•

September 08, 2010

•

Andrei Seluanov, Zhiyong Mao, Vera Gorbunova

¹Department of Biology,University of Rochester

Chapters

00:00Title
01:13Introduction
02:22The Reporter Constructs
05:02Integration of the Reporter Constructs and Induction of DSBs
09:18Analysis of the GFP+ and DsRed+ Cells by Flow Cytometry
11:34Representative FACS Results of the DSB Repair Assay
12:31Conclusion

Summary

자동 번역

本文介绍了基于GFP的荧光<em>在体内</em>分析，分别量化同源重组和非同源末端在哺乳动物细胞中加入。

Tags

DNA Double-strand Break Repair Mammalian Cells Nonhomologous End Joining NHEJ Homologous Recombination HR Perturbations Premature Aging Tumorigenesis Quantitative Measurement Fluorescent Reporter Constructs GFP Gene I-SceI Endonuclease Induction Of DSBs GFP Positive Cells Flow Cytometry

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

使用哺乳动物骨骼肌的DNA转染在体内电穿孔

游离成年哺乳动物元DNA的细胞核内内微量注射

N -连接糖链的糖蛋白在哺乳动物细胞中的脉冲大通分析

异源表达在哺乳动物细胞离子通道的突变和功能分析

雪旺的星形胶质细胞使用相互作用分析<em在体外检测

在哺乳动物细胞通过显微注射mRNA的核出口动力学分析

在哺乳动物细胞中细胞周期位置分析

单工程的RNA转录物的活细胞实时成像采用比例式双分子信标

在联合电子能谱成像miniSOG标记的DNA修复蛋白可视化（ESI）

考试蛋白在哺乳动物细胞中绑定到新生的DNA使用的BrdU芯片插槽西方技术

Read Article