Encyclopedia of Experiments: Biology
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Transcript
Seven weeks after the banding surgery, gently dissect the tissues, adhesions, and fibrosis around the aorta in half of the banded animals until the constriction becomes visible. Carefully dissect the aorta and separate the suture from the vessel. Then, use scissors to cut the suture.
Note that a prolonged occlusion of the ascending aorta during banding or debanding may lead to lung edema and excessive activation of inflammatory pathways.
Close to the chest wall with a simple interrupted or continuous 6-0 polypropylene sutures using the minimum number of stitches possible and close the skin with a 6-0 silk polypropylene suture in a continuous suture pattern. Then, perform the post-operative care as demonstrated.