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Bicyclus anynana의 pupal 날개 조직에서 유전자 발현을 조사하기 위해, 우리는 riboprobes를 사용하여 현장 hybridizations에 대한 최적화된 프로토콜을 제시한다. 우리는 또한 다른 Lepidopteran 종의 pupal 날개에 사용하기 위해이 프로토콜을 더욱 최적화에 대한 지침을 제공합니다.