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单细胞转染鸡胚

JoVE 신문
생물학

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JoVE 신문 생물학

Single Cell Transfection in Chick Embryos

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单细胞转染鸡胚

DOI:

•

08:46 min

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September 25, 2010

•

Raz Ben-Yair, Chaya Kalcheim

¹Department of Medical Neurobiology,Hadassah Medical School – Hebrew University

Chapters

00:00Title
00:57Introduction
01:21Micropipette, DNA, and Embryo Preparation
04:15Loading Micropipettes and Microinjection
06:49Representative Injection Results
08:18Conclusion

Summary

자동 번역

使用精尖微量注入鸡体节或神经管的子域的质粒DNA。质粒的浓度调整，以产生单一的转染细胞。然后，我们允许的细胞发育成无性系种群。

Tags

Single Cell Transfection Chick Embryos 발생학 Lineage Diversification Molecular Mechanisms Transfection Methods Single Progenitors In-ovo Transfection Lineage Tracing Genetic Manipulation Somite Neural Tube Epithelium Sporadic Transfection Clonal Populations Reporters Genetic Manipulation Tools Electroporation Method In-vivo Models

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