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Single Cell Transfektion in Hühnerembryonen

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Single Cell Transfection in Chick Embryos

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Single Cell Transfektion in Hühnerembryonen

DOI:

•

08:46 min

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September 25, 2010

•

Raz Ben-Yair, Chaya Kalcheim

¹Department of Medical Neurobiology,Hadassah Medical School – Hebrew University

Chapters

00:00Title
00:57Introduction
01:21Micropipette, DNA, and Embryo Preparation
04:15Loading Micropipettes and Microinjection
06:49Representative Injection Results
08:18Conclusion

Summary

자동 번역

Mit feiner Spitze Mikropipetten spritzen wir Plasmid-DNA in Subdomains Huhn Somiten oder neuronale Röhren. Die Konzentration der Plasmid wird angepasst, um einzelne transfizierten Zellen zu generieren. Dann erlauben wir die Zellen in klonalen Populationen zu entwickeln.

Tags

Single Cell Transfection Chick Embryos 발생학 Lineage Diversification Molecular Mechanisms Transfection Methods Single Progenitors In-ovo Transfection Lineage Tracing Genetic Manipulation Somite Neural Tube Epithelium Sporadic Transfection Clonal Populations Reporters Genetic Manipulation Tools Electroporation Method In-vivo Models

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