Experiment
JoVE Encyclopedia of Experiments
Encyclopedia of Experiments: Immunology
Encyclopedia of Experiments: Immunology
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Preparation of a Nanoemulsion Adjuvant Vaccine
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Preparation of a Nanoemulsion Adjuvant Vaccine - ConceptTranscript
Begin by mixing 1 milligram of monophosphoryl lipid A with 100 microliters of DMSO. Vortex for five minutes and leave it to dissolve for four hours at room temperature. Quantitatively add Tween 80 and I-OVA. Mix the components and add squalene.
Next, add 100 microliters of MPLA solution to the prepared mixture. To prepare the nanoemulsion vaccine, add the mixed solution to water droplets at approximately 70% of the total volume and stir gently to obtain a transparent and easily flowing mixture.
