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斑马鱼为基础的大型在体内小分子屏幕
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Large Scale Zebrafish-Based In vivo Small Molecule Screen
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斑马鱼为基础的大型在体内小分子屏幕

DOI:
10.3791/2243-v

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07:03 min

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December 30, 2010

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, , ,
1Division of Cardiovascular Medicine, Department of Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology,Vanderbilt University School of Medicine, 4Research Medicine, Veterans Affairs TVHS,Vanderbilt University School of Medicine

Chapters

  • 00:05Title
  • 00:40Introduction
  • 01:02Zebrafish Egg Collection and 96-Well Plate Preparatin
  • 02:52Small Molecule Library
  • 04:23Screening for Effects of Small Molecules by Visual Inspection of Phenotypes
  • 05:51Small Molecule Detection with Zebrafish Chemical Screening Assay
  • 06:21Conclusion

Summary

자동 번역

자동 번역

斑马鱼已成为一个强大的<em>在体内</em>表型为基础的药物屏幕和化学遗传分析平台。在这里，我们展示了一个简单，实用的方法大规模使用斑马鱼的胚胎小分子筛选。

Tags

ZebrafishIn VivoSmall Molecule ScreenPhenotype-based Drug ScreensChemical Genetic AnalysisLarge-scale ScreeningZebrafish Embryos

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