Journal
/
신경과학
/
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
JoVE 신문
신경과학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

DOI:
10.3791/2244-v

•

10:34 min

•

September 17, 2010

•

, , ,
1Neuroscience Center,University of Helsinki, 2Faculty of Biological and Enviromental Sciences,University of Helsinki

Chapters

  • 00:00Title
  • 00:29Method Overview
  • 01:12Introduction
  • 02:30Plasmid Preparation for Electroporation
  • 03:31Glass Needle Preparation
  • 04:07Microinjector Assembling
  • 05:25Anaesthetizing Animal
  • 06:42Surgery, Microinjection, Electroporation
  • 09:22Results
  • 10:19Conclusion

Summary

자동 번역

자동 번역

This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.

Tags

Gene DeliveryPostnatal Rat BrainNon-ventricularPlasmid InjectionElectroporationTransgenic AnimalsGene Of InterestKnockout AnimalsCompensatory MechanismsGene Expression TimingTransfected CellsMicroinjectionIn Vivo ElectroporationTargeted DeliverySiRNADye MoleculesNewborn Rodent BrainNon-migratory Cell TypesTwo-photon In Vivo ImagingElectrophysiological Experiments

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/2244?article_type=v

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
check_url/kr/2244?article_type=v

Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination
check_url/kr/2244?article_type=v

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
check_url/kr/2244?article_type=v

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
check_url/kr/2244?article_type=v

Genoverføring til Developing Mouse indre øret ved<em> In Vivo</em> Electroporation
check_url/kr/2244?article_type=v

Utvidelse av embryonale og voksne nevrale stamceller ved<em> In Utero</em> Electroporation eller Viral stereotaksiske Injection
check_url/kr/2244?article_type=v

In vivo Postnatal Electroporation og Time-lapse Imaging av neuroblast Migration in Mouse Akutt Brain Slices
check_url/kr/2244?article_type=v

Subtype-selektiv Electroporation av Cortical interneuroner
check_url/kr/2244?article_type=v

Analyse av genuttrykk Endringer i Rat Hippocampus Etter dyp hjernestimulering av fremre talamiske Nucleus
check_url/kr/2244?article_type=v

Bruke fluorescensaktivert Cell Sorting å undersøke Cell-Type-Specific genuttrykk i Rat hjernevev

Read Article