Journal
/
Neuroscience
/
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

DOI:
10.3791/50146-v

•

08:44 min

•

September 24, 2013

•

, , , ,
1Department of Neuropathology,Medical School Düsseldorf, 2Department of Molecular Genetics,Weizmann Institute for Science, 3Center of Behavioral Neuroscience,University of Düsseldorf

Chapters

  • 00:05Title
  • 02:01In utero Electroporation (IUE)
  • 03:10Bioluminescence Live Imaging of the Enzymatic Luciferase Reaction
  • 05:47Analysis of Bioluminescence Images
  • 06:41Results: Luminescence Detection After Electroporation
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.

Tags

In Utero ElectroporationBioluminescence ImagingTransgenic RatsDISC1NeurodevelopmentBehavioral Neuroscience

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50146?article_type=v

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
check_url/50146?article_type=v

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
check_url/50146?article_type=v

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
check_url/50146?article_type=v

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
check_url/50146?article_type=v

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
check_url/50146?article_type=v

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
check_url/50146?article_type=v

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
check_url/50146?article_type=v

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
check_url/50146?article_type=v

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
check_url/50146?article_type=v

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

Read Article