JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:12Extract Epithelial Stem Cells from Human Skin
- 03:11Culture Primary Epithelial Stem Cells
- 05:19Immortalize Epithelial Stem Cells
- 06:52Skin Epithelial Stem Cell Culture and Differentiation Results
- 08:02Conclusion
Een snelle, robuuste manier van isoleren levensvatbare volwassen epitheliale stamcellen uit menselijke huid is beschreven. De methode maakt gebruik van enzymatische afbraak van de huid collageen matrix, gevolgd door het plukken van de haarfollikels en isolatie van single cell suspensies of weefsel fragmenten voor celkweek.
Tags
IsolationCultureAdult Epithelial Stem CellsHuman SkinHomeostasisAsymmetric DivisionsDaughter CellsStem Cell PhenotypeTransit-amplifying CellsStem Cell NicheRapid ProliferationTerminal DifferentiationEpidermisHair FollicleIntestineIn Vitro Proliferative PotentialLabel-retaining CellsMulti-potent Stem CellsRodent Hair Follicle Stem CellsEpidermisSebaceous GlandsHair FolliclesHuman Hair Follicle Bulge RegionMulti-potentialityBiomedical Research발생학DifferentiationTumorigenesisTherapeutic Utility
