Journal
/
생물학
/
Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

DOI:
10.3791/2561-v

•

08:26 min

•

March 31, 2011

•

, ,
1Department of Cancer Biology,University of Massachusetts Medical School

Chapters

  • 00:05Title
  • 01:12Extract Epithelial Stem Cells from Human Skin
  • 03:11Culture Primary Epithelial Stem Cells
  • 05:19Immortalize Epithelial Stem Cells
  • 06:52Skin Epithelial Stem Cell Culture and Differentiation Results
  • 08:02Conclusion

Summary

자동 번역

자동 번역

A rapid, robust way of isolating viable adult epithelial stem cells from human skin is described. The method utilizes enzymatic digestion of skin collagen matrix , followed by plucking of hair follicles and isolation of single cell suspensions or tissue fragments for cell culture.

Tags

IsolationCultureAdult Epithelial Stem CellsHuman SkinHomeostasisAsymmetric DivisionsDaughter CellsStem Cell PhenotypeTransit-amplifying CellsStem Cell NicheRapid ProliferationTerminal DifferentiationEpidermisHair FollicleIntestineIn Vitro Proliferative PotentialLabel-retaining CellsMulti-potent Stem CellsRodent Hair Follicle Stem CellsEpidermisSebaceous GlandsHair FolliclesHuman Hair Follicle Bulge RegionMulti-potentialityBiomedical Research발생학DifferentiationTumorigenesisTherapeutic Utility

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts

Isolering af brystepitelceller celler fra tre-dimensionel Blandet-celle klumpformet Co-kultur

Isolering og dyrkning af individuelle myofibre og deres satellit celler fra voksne skeletmuskler

Naive voksne stamceller Isolering fra primære humane fibroblastkulturer

Induktion og analyse af Epithelial til Mesenchymale Transition

Manuel Isolering af Adipose-afledte stamceller fra humane Lipoaspirates

Isolation, Kultur og transplantation af Muskel satellit celler

Isolering og dyrkning af Dental Epiteliale stamceller fra voksne mus Fortand

Read Article