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Neuroscience

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JoVE Journal Neuroscience
Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
 

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons

Article DOI: 10.3791/2568-v • 06:36 min • April 16th, 2011
April 16th, 2011 •
1, 1, 1, 1
1National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda

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FRAP has been used to quantify the mobility of Green Fluorescence Protein (GFP)-tagged proteins in cultured cells. We examined the mobile/immobile fractions of the GFP by analyzing the fluorescence recovery percentage after photobleaching. In this study, FRAP was performed at spines of hippocampal neurons.

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Fluorescence Recovery After Photobleaching (FRAP) GFP-tagged Proteins Dendritic Spines Cultured Hippocampal Neurons Mobility Excitation Laser Fluorescence Recovery Rate Protein Turnover Rate EGFP Vector Zeiss 710 Confocal Microscope Photobleaching Time Lapse Images Mobile Fraction Immobile Fraction ImageJ Software Graphpad Software
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