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使用一种基因编码的钙传感器来监测细胞凋亡过程中线粒体钙水平的动态变化
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생물학
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JoVE 신문 생물학
Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
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使用一种基因编码的钙传感器来监测细胞凋亡过程中线粒体钙水平的动态变化

DOI:
10.3791/2579-v

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06:26 min

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April 01, 2011

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1Department of Neuroscience and Cell Biology,University of Texas Medical Branch

Chapters

  • 00:05Title
  • 01:19Pericam-mt Transfection and Cell Preparation
  • 04:11Image Processing and Analysis
  • 04:37Fluorescence Imaging Results
  • 05:58Conclusion

Summary

자동 번역

자동 번역

本协议描述了线粒体钙通量实时荧光成像测量方法。该方法采用一个基于循环permutated YFP的双激励比例的钙传感器选择性表达在线粒体（比例pericam吨）的优势。

Tags

MonitoringDynamic ChangesMitochondrial Calcium LevelsApoptosisGenetically Encoded Calcium SensorIntracellular Calcium ConcentrationStimuliCellular ProcessesProliferationDifferentiationCalcium AccumulationPro-apoptotic FactorsCytosolLiving CellsIn SituHigh-resolution Fluorescent ImagingDual-excitation RatiometricNon-ratiometric Synthetic Calcium Indicator DyesIntracellular Calcium SignalingCytosolic Calcium FluxesRhod-2Rhod-FFSynthetic IndicatorsMitochondriaRepetitive IncreasesMitochondrial MorphologyLeak Out Of MitochondriaLong-term ExperimentsGenetically Encoded Calcium IndicatorsGreen Fluorescent Protein (GFP)Aequorin

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