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确定远距离的DNA相互作用的相关染色体陷阱

JoVE 신문
생물학

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JoVE 신문 생물학

Associated Chromosome Trap for Identifying Long-range DNA Interactions

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确定远距离的DNA相互作用的相关染色体陷阱

DOI:

•

14:49 min

•

April 23, 2011

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Jianqun Ling, Andrew R. Hoffman

¹Medical Service, VA Palo Alto Health Care System ,Stanford University School of Medicine

Chapters

00:05Title
01:30Formaldehyde Fixation of Long-Range Chromatin Interactions and Nuclei Isolation
03:36Digestion, Ligation and Purification of Interaction DNA Segments
06:10Digestion with Msp I and Ligation with Oligonucleotide Linkers
07:50PCR Amplification and Sequence Analysis
11:06Act Assay Representative Results
14:23Conclusion

Summary

자동 번역

相关的染色体陷阱（ACT）的分析是用于识别远射DNA相互作用的一种新的不带偏见的方法。远距离DNA相互作用的特性，将使我们在正常的生理和患病状态，以确定核架构的关系，基因的表达。

Tags

Associated Chromosome Trap Long-range DNA Interactions Chromatin Structure Chromosome Territories Gene Expression Transcriptional Factories Co-localization ACT Assay Chromosome Conformation Capture Technique Transcriptional Regulation Nuclear Architecture Gene Expression In Disease States

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