This content is Free Access.
Chapters
- 00:00Title
- 00:13Introduction
- 00:41Tail Vein Injection for Adoptive Transfer of CFSE-labelled T Lympho
- 03:45Removal of Lymph Nodes
- 06:42Removing the Inguinal Lymph Nodes
- 08:48Removing the Axillary Lymph Nodes
- 11:24Removing the Cervical Lymph Nodes
- 13:07Preparation of Lymph Nodes for In Situ Imaging
- 15:362-photon Imaging of Lymph Nodes in Situ
Dois fótons de imagem revelou motilidade linfócitos e interações celulares dentro do nó de linfa em condições basais e durante uma resposta imune 1. Aqui, demonstramos transferência adotiva de células T, o isolamento dos gânglios linfáticos, e motilidade de imagens de células CD4 + T no linfonodo explantado.
Tags
Dissection2-Photon ImagingPeripheral Lymph NodesMiceAdoptive TransferLabeled T CellsIsolationImaging MotilityCD4+ T CellsExplanted Lymph NodeTwo-photon Imaging Of Immune CellsFluorescently Labeled CellsCell Tracker DyeFluorescent ProteinTail Vein InjectionLymphoid OrgansIsolation Of Lymph NodeExcised Lymph NodeOxygenation Of Perfused MediaTemperatureLaser Power3D Video ImagesNaive CD4+ T Cells
