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发展中国家斑马鱼的表皮细胞挤压实时成像
JoVE 신문
생물학
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JoVE 신문 생물학
Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish
DOI:

09:39 min

June 27, 2011

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Chapters

  • 00:05Title
  • 01:59Injection of mRNA Encoding the Fluorescently-tagged Actin Binding Protein
  • 03:47Induction of Cell Extrusion in Zebrafish Larvae Using G418
  • 04:47Mounting Zebrafish Larvae for Imaging
  • 05:34Live Imaging of Actin Dynamics and Individual Epithelial Cell Behaviors during Cell Extrusion Using a Spinning Disc Confocal Microscope
  • 08:22Representative Epidermal Images
  • 08:55Conclusion

Summary

자동 번역

死亡的细胞被挤压协调一致的收缩邻近的细胞,上皮组织,而不破坏的屏障功能。发展中国家斑马鱼的光学清晰度提供了一个极好的系统,以可视化挤出生活上皮。在这里,我们描述的方法来诱导和幼虫斑马鱼的表皮细胞决议形象挤压。

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