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Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
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1Institute for Memory Impairments and Neurological Disorders,University of California, 2Physical Medicine & Rehabilitation,University of California, 3Anatomy & Neurobiology,University of California, 4Sue and Bill Gross Stem Cell Research Center,University of California, 5Section of Molecular Biology,University of California, 6Reeve-Irvine Research Center,University of California
Chapters
- 00:05Title
- 01:23Dissociation of Spinal Cord Tissue
- 02:47Creating OptiPrep Gradient Solutions and Separating Lipid/Myelin Debris from Cells
- 04:27Immunolabeling of Specific Immune Cells and Flow Cytometric Quantitative Assessment
- 06:16Removal of Lipid/Myelin Debris Enhances Quantitative Detection of Cellular Inflammation in the Injured Spinal Cord Tissue by Flow Cytometry
- 07:33Conclusion
Quantification of cellular inflammation in the injured/pathological CNS by flow cytometry is complicated by lipid/myelin debris that can have similar size and granulation to cells, decreasing sensitivity/accuracy. We have advanced a cell preparation method to remove myelin debris and improve cell detection by flow cytometry in the injured spinal cord.
Tags
Quantitative AssessmentImmune CellsFlow CytometryCell Purification MethodCentral Nervous System (CNS)Cellular InflammationMorphological TechniquesHistological TechniquesAlternative MethodMyelin DebrisOptiPrep Gradient SystemSensitive QuantificationsReliable QuantificationsInjury SeverityCharacterization
