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A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells
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Chapters
- 00:05Title
- 02:14Perfusion of the Liver
- 03:33Preparation and Culture of Parenchymal Hypatocyte-rich Cell Fraction
- 04:36Selective Isolation of Macrophages
- 06:46Results: Highly-purified Rat Liver Macrophages
- 07:22Conclusion
A novel method to obtain macrophages from primary culture of rat liver cells is described. This method utilizes the proliferation of macrophages in the culture, followed by shaking of culture flasks and purification by selective attachment to plastic dishes. This technique efficiently provides liver macrophages without complex equipment and skills.
Tags
Isolate MacrophagesMixed Primary CulturesAdult Liver CellsKupffer CellsLiver-specific Resident MacrophagesIsolation MethodsSophisticated EquipmentCentrifugal ElutriatorTechnical SkillsNovel MethodSufficient Number And PurityRat Liver CellsTwo-step Perfusion MethodParenchymal HepatocytesCulture MediumDMEMFCSEpithelial Cell MorphologyFibroblast-like CellsPhase Contrast-bright Round Macrophage-like CellsProliferation Of Macrophage-like CellsShaking Of Culture FlasksSuspension Of MacrophagesTransfer And Short Incubation In Plastic Dishes
