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שיטה פשוטה ויעילה על מנת לבודד המקרופאגים מתרבויות ראשי מעורבות של תאים בכבד למבוגרים
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Chapters
- 00:05Title
- 02:14Perfusion of the Liver
- 03:33Preparation and Culture of Parenchymal Hypatocyte-rich Cell Fraction
- 04:36Selective Isolation of Macrophages
- 06:46Results: Highly-purified Rat Liver Macrophages
- 07:22Conclusion
Tags
Isolate MacrophagesMixed Primary CulturesAdult Liver CellsKupffer CellsLiver-specific Resident MacrophagesIsolation MethodsSophisticated EquipmentCentrifugal ElutriatorTechnical SkillsNovel MethodSufficient Number And PurityRat Liver CellsTwo-step Perfusion MethodParenchymal HepatocytesCulture MediumDMEMFCSEpithelial Cell MorphologyFibroblast-like CellsPhase Contrast-bright Round Macrophage-like CellsProliferation Of Macrophage-like CellsShaking Of Culture FlasksSuspension Of MacrophagesTransfer And Short Incubation In Plastic Dishes
