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从细胞中一氧化氮和超氧负离子自由基的电子顺磁共振光谱的检测，使用自旋陷阱
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JoVE 신문 생물학
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
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从细胞中一氧化氮和超氧负离子自由基的电子顺磁共振光谱的检测，使用自旋陷阱

DOI:
10.3791/2810-v

•

13:21 min

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August 18, 2012

•

, , ,
1The Davis Heart and Lung Research Institute,The Ohio State University, 2Department of Pharmacology,College of Medicine, The Ohio State University

Chapters

  • 00:05Title
  • 00:09Introduction
  • 02:56Detection of Nitric Oxide (NO) with Culture of Bovine Aortic Endothelial Cells (BAEC)
  • 05:56Endothelial NOS (eNOS) Uncoupling Experiment using SIN-1
  • 08:12Detection of superoxide (O2–) from polymorphonuclear neutrophils (PMNs)
  • 10:20Conclusion

Summary

자동 번역

자동 번역

采用电子顺磁共振（EPR）光谱检测牛主动脉内皮细胞和人类使用铁（I​​I）-N-甲基-D葡萄糖胺二硫代氨基甲酸铁（百万加仑）中性粒细胞超氧阴离子自由基一氧化氮<sub> 2</sub>和5,5 - 二甲基-1-pyroroline - N-氧化物，DMPO分别。

Tags

DetectionNitric OxideSuperoxide Radical AnionElectron Paramagnetic Resonance SpectroscopyCellsSpin TrapsReactive Nitrogen SpeciesReactive Oxygen SpeciesCell FunctionImmune ResponseAntioxidant Defense MechanismsOxidative DamageProtein FunctionDNA CleavageLipid PeroxidationOxidative StressCell InjuryCell DeathSuperoxide Radical Anion GenerationPeroxynitriteHydroxyl RadicalPolymorphonuclear NeutrophilsChemotactic StimulationPhorbol-12-myristate-13-acetateNicotinamide Adenine Dinucleotide Phosphate OxidaseNitric Oxide Synthase

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