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Chapters
- 00:05Title
- 01:10Expression and Extraction of the Proteins
- 03:22Immobilization of ProIM and ProIMnc on the Membrane
- 03:54Re-folding of Membrane-bound Proteins
- 04:49Probing the ProIM by ProSOL
- 05:33Visualizing Protein-protein Interaction by Immunoblotting
- 07:06Representative Results
- 08:20Conclusion
Testing protein-protein interaction is indispensable for dissection of protein functionality. Here, we introduce an in vitro protein-protein binding assay to probe a membrane-immobilized protein with a soluble protein. This assay provides a reliable method to test interaction between an insoluble protein and a protein in solution.
