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1Department of Orthodontics and Maxillofacial Orthopedics,Medical University of Graz, 2Institute of Hygiene, Microbiology and Environmental Medicine,Medical University of Graz, 3Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology,Medical University of Graz, 4Institute of Plant Sciences,Karl-Franzens-University Graz
Chapters
- 00:05Title
- 01:51Specimen Collection and Biofilm Fixation
- 03:38Dehydration of Fixed Samples
- 04:34In-situ Hybridization and Imaging
- 07:21Results: Biofilm Images
- 08:56Conclusion
Nós apresentamos um protocolo para análise estrutural e de composição do biofilme oral natural de aparelhos ortodônticos com<em> In situ</em> Hybridization (FISH) e microscopia de varredura confocal a laser (CLSM). Amostras de biofilme oral foram coletados de expansores palatinos, raspagem de resina acrílica flocos de fora de sua superfície e encaminhá-los para o processamento molecular.
