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Immunology and Infection

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JoVE Journal Immunology and Infection
Ex vivo beeldvorming van T-cellen in muizen lymfkliertest Plakjes met Widefield en confocale microscopen
 
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Ex vivo beeldvorming van T-cellen in muizen lymfkliertest Plakjes met Widefield en confocale microscopen

Article DOI: 10.3791/3054-v • 09:46 min • July 15th, 2011
July 15th, 2011 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Institut Cochin, Université Paris Descartes, CNRS (UMR 8104), 2Inserm, U1016, Paris, France

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Dit protocol beschrijft een methode om de afbeelding fluorescerende T-cellen geïntroduceerd in lymfeklier plakjes. De techniek maakt real-time analyses van T-cel migratie met traditionele groothoek fluorescentie of confocale microscopen.

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Immunologie muis lymfeklieren organotypische plakken T-cel migratie fluorescentie microscopie confocale
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