Journal
/
신경과학
/
Neural Tube Closure in Mouse Whole Embryo Culture
JoVE 신문
신경과학
This content is Free Access.
JoVE 신문 신경과학
Neural Tube Closure in Mouse Whole Embryo Culture

Neural Tube Closure in Mouse Whole Embryo Culture

DOI:
10.3791/3132-v

•

07:37 min

•

October 21, 2011

•

,
1Department of Neurology/Neuroscience,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 01:47Preparation of Culture Medium and Isolation of Uterus and Removal of Embryos
  • 03:59Setting up the Culture System
  • 05:20Evaluating Development after Whole Embryo Culture
  • 06:10Representative Embryo Cultures
  • 07:08Conclusion

Summary

자동 번역

자동 번역

A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.

Tags

Neural Tube ClosureMouse EmbryosEmbryo CultureGenetic Mouse ModelsMammalian Neural Tube ClosureMaternal MetabolismPharmacological ManipulationSmall Molecule DeliveryRecombinant Protein DeliverySiRNA DeliveryWhole Embryo CultureEnriched MediaRoller Incubator ApparatusNormal Neural Tube ClosureIn Vitro TechniquesNeurulationNeural Plate FormationCranial Fold ClosureCaudal Neuropore ClosureEmbryo Dissection

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/3132?article_type=v

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
check_url/kr/3132?article_type=v

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making
check_url/kr/3132?article_type=v

Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
check_url/kr/3132?article_type=v

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
check_url/kr/3132?article_type=v

Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology
check_url/kr/3132?article_type=v

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence
check_url/kr/3132?article_type=v

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis
check_url/kr/3132?article_type=v

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
check_url/kr/3132?article_type=v

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
check_url/kr/3132?article_type=v

Chicken Embryo Spinal Cord Slice Culture Protocol

Read Article