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1Department of Computer Science and Engineering,Texas A&M University, 2Beckman Institute for Advanced Science and Technology,University of Illinois, 3Department of Electrical and Computer Engineering,Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences,Texas A&M University
Chapters
- 00:05Title
- 01:27Specimen Preparation: Golgi-Cox Method
- 03:20Specimen Preparation: Nissl Method
- 04:39Specimen Preparation: India Ink Method
- 05:37KESM Setup and Imaging
- 06:38Image Processing, Data Visualization and Analysis
- 08:12Representative Results of KESM
- 10:00Conclusion
Le processus complet de préparation des échantillons à l'imagerie cérébrale coupes sériées en utilisant le microscope à effet couteau, à la visualisation de données et d'analyse est décrite. Cette technique est actuellement utilisée pour acquérir des données cerveau de souris, mais il est applicable à d'autres organes, d'autres espèces.
Tags
Specimen PreparationImagingAnalysis ProtocolsKnife-edge Scanning MicroscopyHigh-throughput MicroscopyHigh-resolution Microscopy3D MicroscopyNeuroanatomical DataSubmicrometer ResolutionWhole-brain-scale DataNeuronal NetworksVascular NetworksCell Body DistributionConnectomics ResearchMicrocirculation ResearchHemodynamic ResearchStereology ResearchFixingStainingEmbeddingKESM Configuration And SetupSectioning And Imaging With KESMImage ProcessingData PreparationData VisualizationData Analysis
