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Chapters
- 00:01Title
- 00:04Introduction
- 00:58Cell Culture Preparation for the Experiment
- 01:42Hardware Equipment
- 02:09Gene Electrotransfer Protocol
- 03:31Image Acquisition and Determination of Gene Electrotransfer Efficacy
Gene transfection by electroporation is improved approximately two times when orientation of electric field is changed during pulse application, while cell viability is not affected. The increase in gene transfection is caused by the increase of the membrane area which is made competent for DNA entry into the cell.
