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Immunology and Infection

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JoVE Journal Immunology and Infection
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
 

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Article DOI: 10.3791/3321-v • 06:38 min • November 8th, 2011
November 8th, 2011 •
1,2, 1,2, 2, 2, 1,2,3,4
1Stony Brook Children's Hospital, State University of New York at Stony Brook, 2Department of Pediatrics, State University of New York at Stony Brook, 3Department of Molecular Genetics, State University of New York at Stony Brook, 4Department of Microbiology, State University of New York at Stony Brook

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요약
Automatic Translation

We describe a method for generating transformed B cell lines using Epstein-Barr virus. We also illustrate a novel assay that can identify B cells destined to undergo transformation as early as three days after infection.

Tags

Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines EBV Infection B Cell Proliferation Immortalization EBV Latency Virus-driven B Cell Proliferation Tumorigenesis Immunologic Assays Human Monoclonal Antibodies Mitogens Phytohemagglutinin Lipopolysaccharide Pokeweed Mitogen Cyclosporin A T Cell-mediated Killing High Titer EBV Immunosuppressive Agent Peripheral Blood Mononuclear Cells
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