Journal
/
면역학 및 감염병학
/
在复杂的临床和环境样本使用寡核苷酸加上荧光微多元检测细菌
JoVE 신문
면역학 및 감염병학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Please note that all translations are automatically generated. Click here for the English version.

在复杂的临床和环境样本使用寡核苷酸加上荧光微多元检测细菌

DOI:
10.3791/3344-v

•

11:09 min

•

October 23, 2011

•

, , , ,
1Saskatoon Research Centre,Agriculture and Agri-Food Canada, 2Department of Veterinary Microbiology,University of Saskatchewan , 3Plant Biotechnology Institute,National Research Council of Canada

Chapters

  • 00:05Title
  • 03:05Coupling Oligonucleotide Probes to Fluorescent Beads
  • 06:37Production of cpn60 Universal Target Amplicon and Generation of Single Strands
  • 09:36Representative Results

Summary

자동 번역

자동 번역

我们描述了一个复用的微生物样本内使用寡核苷酸耦合的荧光珠的检测方法。从样本内的所有生物体的扩增杂交的探针耦合珠小组。一个Luminex公司或生物复杂的仪器是用于查询每个珠珠型和杂交信号。

Tags

Multiplex DetectionBacteriaClinical SamplesEnvironmental SamplesOligonucleotide-coupled Fluorescent MicrospheresBacterial VaginosisPolymicrobial SyndromeLactobacillusGardnerella VaginalisAtopobium VaginaeNegative Health OutcomesHIV AcquisitionClinical ManagementDiagnosisGram StainsVaginal Swab SmearsSubjective InterpretationsVaginal Microbiota CompositionDeep SequencingMolecular Diagnosis

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

使用泛病毒基因芯片检测（Virochip）屏幕病毒病原体的临床标本

使用荧光免疫化学检测爱德华ictaluri（一毛虫）样品

确定临床抗体样品的吞噬活性

检测使用荧光脱氧核酶的细菌

革兰从血培养液使用MALDI-TOF质谱阴性细菌快速鉴定

原位检测自身反应性CD4 + T细胞在大脑和心脏使用主要组织相容性复合II类Dextramers的

原位检测使用地高辛标记的DNA探针石蜡包埋组织中的细菌靶向16S rRNA基因

一种快速战略新Faustoviruses从环境样品使用隔离<em> Vermamoeba vermiformis</em

制定唾液抗体多重免疫测定来测量人体暴露于环境中的病原体

对于检测磁力搅拌器方法<em>旋毛虫</em>幼虫肌肉样品

Read Article