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Chapters
- 00:05Title
- 01:39Preparation of the Electrostatic Chamber
- 02:54Neural Progenitor Cell Isolation
- 04:09Application of the Electric Field to the Electrostatic Chamber
- 07:18Preparation of the Organotypic Spinal Cord Slice
- 08:40Injection of Hoechst 33342 Labeled Neural Progenitor Cells into the Organotypic Spinal Cord Slice
- 09:38Results: Neural Progenitor Cells Show Directed Migration in the Electric Field and the Organotypic Spinal Cord Slice
- 10:46Conclusion
This protocol demonstrates methods used to establish 2D and 3D environments in custom-designed electrotactic chambers, which can track cells in vivo/ex vivo using time-lapse recording at the single cell level, in order to investigate galvanotaxis/electrotaxis and other cellular responses to direct current (DC) electric fields (EFs).
