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使用光声血流循环黑色素瘤细胞的检测和隔离
JoVE 신문
생체공학
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JoVE 신문 생체공학
Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
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使用光声血流循环黑色素瘤细胞的检测和隔离

DOI:
10.3791/3559-v

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09:52 min

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November 25, 2011

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, , , , , ,
1Biological Engineering, Christopher S. Bond Life Sciences Center, Dermatology,University of Missouri

Chapters

  • 00:05Title
  • 01:20Blood Sample Preparation
  • 02:58Flow Chamber Construction
  • 04:32Photoacoustic Flowmetry Set-up
  • 06:42Immunocytochemistry
  • 08:39Results: Melanoma Isolation
  • 09:30Conclusion

Summary

자동 번역

자동 번역

我们已经开发出一种使用激光诱导超声检测循环黑色素瘤细胞的转移性疾病的早期指标的流式细胞仪。

Tags

DetectionIsolationCirculating Melanoma CellsPhotoacoustic FlowmetryCirculating Tumor Cells (CTCs)Metastatic DiseaseBlood SamplesCancer DiagnosisTherapy Response MonitoringPigmentPhotoacoustic WavesCustom Flow CytometerSeparationCentrifugationWhite Blood Cell LayerDensity SimilarityPhosphate Buffered Saline (PBS)FlowmeterTwo Phase FlowMicrofluidic SystemSlugs Of LiquidLaser Light Irradiation

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