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Mess-Peptide Translokation in große unilamellare Vesikel
JoVE 신문
생물학
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JoVE 신문 생물학
Measuring Peptide Translocation into Large Unilamellar Vesicles
DOI:

12:27 min

January 27, 2012

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Chapters

  • 00:05Title
  • 01:37Preparation of Large Unimellar Lipid Vesicles (LUVs)
  • 05:01Quantifying LUV Concentration
  • 08:05Preparing Peptide Solutions
  • 08:46Translocation Quantification
  • 10:37Results: Translocation Assay
  • 11:58Conclusion

Summary

자동 번역

Dieses Protokoll Details eine Methode zur quantitativen Messung von Peptid-Translokation in große unilamellare Lipidvesikel. Diese Methode liefert auch Informationen über die Geschwindigkeit der Membran-Translokation und kann verwendet werden, um Peptide, die effizient und spontan Kreuz Lipid-Doppelschichten zu identifizieren.

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