Journal
/
생물학
/
Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach

Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach

DOI:
10.3791/3759-v

•

08:29 min

•

March 27, 2012

•

,
1Department of Bioengineering,University of Pittsburgh, 2Department of Chemical Engineering,University of Pittsburgh

Chapters

  • 00:05Title
  • 01:20Culturing Human Embryonic Stem Cells
  • 02:05Definitive Endoderm and Pancreatic Progenitor Induction
  • 03:01Pancreatic Maturation
  • 03:57qRT-PCR Analysis
  • 05:06Immunocytochemistry
  • 06:21Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
  • 08:07Conclusion

Summary

자동 번역

자동 번역

The current study describes a directed differentiation approach in inducing pancreatic differentiation of human embryonic stem cells. Of great significance is the finding that endothelial cell co-culture mediates maturation of human embryonic stem cell derived pancreatic progenitors into insulin expressing cells.

Tags

Endothelial Cell Co-cultureHuman Embryonic Stem CellsDirected Differentiation ApproachPancreatic Insulin Producing CellsEmbryonic Stem CellsPluripotentCell Based TherapyRegenerative TreatmentCellular DifferentiationOrganogenesisPancreas DevelopmentEndoderm InductionNodal Pathway ModulationActivin AGrowth FactorsSonic Hedgehog InhibitionCyclopaminePancreatic MaturationNotch Signaling InhibitionVascularization

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/3759?article_type=v

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
check_url/kr/3759?article_type=v

Derivation of Human Embryonic Stem Cells by Immunosurgery
check_url/kr/3759?article_type=v

Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia
check_url/kr/3759?article_type=v

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
check_url/kr/3759?article_type=v

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
check_url/kr/3759?article_type=v

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
check_url/kr/3759?article_type=v

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
check_url/kr/3759?article_type=v

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
check_url/kr/3759?article_type=v

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
check_url/kr/3759?article_type=v

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

Read Article