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Chapters
- 00:05Title
- 00:56Egg Handling and Needle Preparation
- 03:20Injection and Electroporation
The overall goal of this video is to show how to perform targeted retinal injection and in ovo electroporation of DNA/RNA constructs into the chick embryonic retina at the Hamburger and Hamilton stage 22-23, which is about embryonic day 4 (E4). This technique is very useful to study gene expression, gene regulation, and morphological change in developing chick retina.
Tags
Ovo ElectroporationEmbryonic Chick RetinaRetinal DevelopmentGene RegulationRecombinant DNA/RNA TechnologyReporter Gene AssayGene Over-expressionGene Knock DownTargeted Retinal InjectionHamburger And Hamilton Stage 22-23Embryonic Day 4 (E4)Green Fluorescent Protein (GFP)Subretinal SpaceRetinal Stem/progenitor CellsLate-born Neurons
