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Immunology and Infection

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JoVE Journal Immunology and Infection
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
 

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Article DOI: 10.3791/3892-v • 16:10 min • March 22nd, 2012
March 22nd, 2012 •
1, 1, 1
1Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health

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요약
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The compartmentalization of proteins either within the plasma membrane or into intracellular locations is one regulatory mechanism that can greatly influence signaling outcomes; hence, to understand signaling it is important to study the spatial and temporal behavior of the proteins involved. We describe here a TIRF microscopy based system to study signal transduction in T cells, but is broadly applicable.

Tags

TIRF Microscopy Real-time Imaging TCR Signaling Proteins Antigen Presenting Cells PMHC Proteins TCR Complex CD3 Dimers Src Family Kinase Lck Syk Family Kinase Zap70 Adapter Proteins LAT SLP76 Proximal Signaling Complex Calcium-dependent Transcription Factor Activation Gene Expression Programs T Cell Differentiation Antigen Potency Co-stimulatory Receptors Co-inhibitory Receptors Chemokine Receptors Cytokine Receptors
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