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JoVE Journal Neuroscience
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
 
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Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Article DOI: 10.3791/3907-v • 09:51 min • July 15th, 2012
July 15th, 2012 •
1, 1,2, 1
1Fishberg Department of Neuroscience and Friedman Brain Institute, Mt. Sinai School of Medicine, 2Phase Five Communications Inc.

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We demonstrate how changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. We image intracellular calcium using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.

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Intracellular Calcium Synaptic Efficacy Mollusc Aplysia Calcium Imaging Intracellular Recording Buccal Ganglion Synaptic Plasticity Homosynaptic Facilitation Calcium Orange Iontophoresis Fluorescent Dye
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