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优化小,解剖定义的大脑区域的DNA甲基化和基因表达分析
JoVE 신문
신경과학
This content is Free Access.
JoVE 신문 신경과학
Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
DOI:

13:11 min

July 12, 2012

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Chapters

  • 00:05Title
  • 01:58Programming by Early Life Adversity
  • 02:45Collecting Brain Tissue Punches
  • 04:02Nucleic Acid Extraction from Brain Punches
  • 06:08Bisulfite PCR
  • 07:41Bisulfite Sequencing
  • 10:57The Effect of ELS on the Expression and Methylation of AVP
  • 12:35Conclusion

Summary

자동 번역

一个精简的工作流程,研究DNA甲基化和基因表达变化后早期生活压力。从新生鼠和离散脑组织隔离母体分离开始,我们代表的协议,同时为随后的亚硫酸氢钠测序和RT-PCR分析DNA和RNA分离脑组织拳。

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