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JoVE Journal Neuroscience
In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development
 

In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development

Article DOI: 10.3791/4017-v • 08:57 min • August 3rd, 2012
August 3rd, 2012 •
*1, *1, 1,2
1Northwestern University Feinberg School of Medicine, Children's Hospital of Chicago Research Center, 2Departments of Pediatrics, Neurology and Physiology, Northwestern University Feinberg School of Medicine
* These authors contributed equally

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요약
Automatic Translation

To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation.

Tags

Ovo Electroporation Chick Midbrain Gene Function Dopaminergic Neuron Development Parkinson's Disease Schizophrenia Depression Dementia Neural Progenitors Gene Expression Programs Experimental Manipulations Chick Embryonic Neural Tubes PCAG Vector CMV Promoter
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