JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:22Oocyte Collection
- 03:09Agarose Embedding and Lysis
- 04:41Bisulfite Mutagenesis
- 07:00First and Second Round PCR Amplification
- 09:09TA Cloning and Colony PCR
- 10:31Results: Electrophoretic Analysis of Single Oocyte Bisulfite Mutagenesis
- 12:40Conclusion
Tags
Single Oocyte Bisulfite MutagenesisEpigeneticsChromatin ModificationsGene AccessibilityGene TranscriptionDNA MethylationRepressive MarkCpG DinucleotidesMethyl GroupRepressive ProteinsHistone ModificationsDevelopmental ProgrammingCell DifferentiationRetroviral ElementsX-chromosome InactivationGenomic ImprintingBisulfite MutagenesisSodium BisulfiteDeaminationUracilsPCR AmplificationSequencingConversion EventsThyminesMethylated CytosinesNucleotide LevelAgarose Bead
