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Chapters
- 00:05Title
- 01:27Construction of Environmental Chambers
- 02:56Connecting the Gas Supply to the Environmental Chamber
- 04:39Preparation of Samples for Viability Assay
- 07:32Rapid Harvest of Hypoxia-exposed Worms
- 10:11Results: The Effect of Hypoxia on C. elegans
- 10:44Conclusion
This paper details how to use continuous-flow hypoxia chambers to generate atmospheres with defined concentrations of O2 to understand biological responses to decreased O2. This system is easy to setup and maintain, and flexible enough to suit a wide range of O2 concentrations and model systems
