Journal
/
신경과학
/
Automated Quantification of Synaptic Fluorescence in C. elegans
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Automated Quantification of Synaptic Fluorescence in C. elegans

Automated Quantification of Synaptic Fluorescence in C. elegans

DOI:
10.3791/4090-v

•

12:22 min

•

August 10, 2012

•

,
1Department of Biological Sciences,University of Toledo

Chapters

  • 00:05Title
  • 01:29Preparation of Worms for Imaging
  • 04:50Confocal Imaging
  • 07:34Automated Identification and Analysis of Individual Synaptic Clusters
  • 10:28Representative Results
  • 11:58Conclusion

Summary

자동 번역

자동 번역

The abundance of neurotransmitter receptors clustered at synapses strongly influences synaptic strength. This method quantifies fluorescently-labeled neurotransmitter receptors in three dimensions with single-synapse resolution in C. elegans, allowing hundreds of synapses to be rapidly characterized within a single sample without distortions introduced by z-plane projection.

Tags

Automated QuantificationSynaptic FluorescenceC. ElegansSynapse StrengthNeurotransmitter ReceptorsSynaptic SitesPostsynaptic MembraneReceptor TraffickingSynaptic PlasticityNeuromodulationFluorescence MicroscopySynaptically-localized Neurotransmitter Receptor AbundanceSynaptic DevelopmentSynaptic PlasticityCaenorhabditis ElegansNative SynapsesAutomated IdentificationThree Dimensions

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/4090?article_type=v

Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
check_url/kr/4090?article_type=v

In vivo Laser Axotomy in C. elegans
check_url/kr/4090?article_type=v

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
check_url/kr/4090?article_type=v

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
check_url/kr/4090?article_type=v

C. elegans Tracking and Behavioral Measurement
check_url/kr/4090?article_type=v

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans
check_url/kr/4090?article_type=v

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
check_url/kr/4090?article_type=v

In vivo Neuronal Calcium Imaging in C. elegans
check_url/kr/4090?article_type=v

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
check_url/kr/4090?article_type=v

In Vivo Imaging of Dauer-specific Neuronal Remodeling in C. elegans

Read Article