JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:29Preparation of Worms for Imaging
- 04:50Confocal Imaging
- 07:34Automated Identification and Analysis of Individual Synaptic Clusters
- 10:28Representative Results
- 11:58Conclusion
De overvloed aan neurotransmitter receptoren geclusterd bij synapsen grote invloed op synaptische sterkte. Deze methode kwantificeert fluorescent gelabelde neurotransmitter receptoren in drie dimensies met single-synaps resolutie in<em> C. elegans</em>, Waardoor honderden synapsen snel worden gekenmerkt binnen een monster zonder misvormingen die z-vlak projectie.
Tags
Automated QuantificationSynaptic FluorescenceC. ElegansSynapse StrengthNeurotransmitter ReceptorsSynaptic SitesPostsynaptic MembraneReceptor TraffickingSynaptic PlasticityNeuromodulationFluorescence MicroscopySynaptically-localized Neurotransmitter Receptor AbundanceSynaptic DevelopmentSynaptic PlasticityCaenorhabditis ElegansNative SynapsesAutomated IdentificationThree Dimensions
