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Chapters
- 00:05Title
- 02:47Preparation of Aggresome Donor Cells: Transfection of mRFP- or eGFP-tagged Human Full-length DISC1
- 03:50Aggresome Purification from Transfected Donor Cells
- 06:43Treatment of Recipient SH-SYBY Cells with mRFP-DISC1 Aggresomes
- 08:13Labeling of Recombinant DISC1(598-785) with DyLight594
- 10:44Treatment of Recipient SH-SY5Y Cells with Labeled Recombinant DISC1(598-785) Protein
- 12:00Representative Invasion Assay Results
- 13:30Conclusion
La generación, purificación y la invasión celular de aggresomes intracelulares, citoplasmáticas de longitud completa de proteína DISC1 de cultivos celulares y de un marcado, multimérica fragmento de la proteína recombinante en DISC1<em> E. coli</em> Se describen. Invasividad de las células se muestra para las células receptoras en cultivo celular y para las neuronas<em> In vivo</em> Después de la inoculación cerebro estereotácticos.
Tags
Protein AggregationChronic Degenerative Brain ConditionsNeurodegenerative DiseasesAlzheimer’s DiseaseTauParkinson’s DiseaseAlpha-synucleinHuntington’s DiseasePolyglutamineHuntingtinProteostasisMisfolded ProteinsSporadic FormsFamilial FormsSchizophreniaCognitive DeficitDisrupted-in-schizophrenia 1 (DISC1)Scottish FamilyChronic Mental DiseaseInsoluble AggregatesSMRI CCSarkosyl-insoluble DISC1 ImmunoreactivityBiochemical FractionationDisease-associated Polymorphism S704CDISC1 Aggresomes
