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Immunology and Infection

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JoVE Journal Immunology and Infection
A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
 

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Article DOI: 10.3791/4177-v • 12:11 min • July 9th, 2012
July 9th, 2012 •
1, 2, 2, 3, 1
1National Exposure Research Laboratory, Office of Research and Development, US Environmental Protection Agency, 2Shaw Environmental & Infrastructure, 3Office of Ground Water and Drinking Water, US Environmental Protection Agency

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This protocol describes the use of a tangential flow hollow-fiber ultrafiltration sample concentration system and a heat dissociation as alternative steps for the detection of waterborne Cryptosporidium and Giardia species using EPA Method 1623.

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Modified EPA Method 1623 Tangential Flow Hollow-fiber Ultrafiltration Heat Dissociation Waterborne Cryptosporidium Giardia Spp. Protozoa Diarrheal Disease Outbreaks Prevalence Monitoring US Drinking Water Supplies Sample Concentration Immunomagnetic Separation Procedure Acid Dissociation Procedure Immunofluorescence Staining And Enumeration Envirochek Filters Envirochek HV Filters Filta-Max Filters
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