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Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
 

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

Article DOI: 10.3791/4198-v • 14:02 min • November 1st, 2012
November 1st, 2012 •
1, 2, 1, 2, 1, 1,3
1Department of Physiology and Biophysics, UMDNJ-Robert Wood Johnson Medical School, 2Muscle Biology Research Group, University of Missouri-Kansas City, 3Pharmacology division, College of Pharmacy, DHLRI, Ohio State University

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요약
Automatic Translation

We describe a method to directly measure muscle force, muscle power, contractile kinetics and fatigability of isolated skeletal muscles in an in vitro system using field stimulation. Valuable information on Ca2+ handling properties and contractile machinery of the muscle can be obtained using different stimulating protocols.

Tags

Ex Vivo Assessment Contractility Fatigability Alternans Isolated Skeletal Muscles Muscle Force Muscle Power Contractile Kinetics Recovery After Fatigue Excitation-contraction Coupling (ECC) Excitability Contractile Machinery Ca2+ Handling Ability Genetic Components Ca2+ Signaling Pathways Skeletal Muscle Phenotype In Vitro Muscle Contractility Assay Single Cell Assays Genetic Approaches Biochemistry Assays Mechanisms Of ECC
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