JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:54Primary NOF Cell Line: Overexpressing hTERT
- 04:15PolyHEMA Coating of Cell Culture Dishes
- 05:07Generating 3D Heterotypic Spheroids
- 06:43Re-feeding of 3D Cultures
- 07:58Processing for Imaging and Molecular Analyses
- 11:36Conclusion
Se describen las metodologías para el establecimiento de<em> In vitro</em> Heterotípicas modelos tridimensionales que comprenden fibroblastos de ovario y de superficie ovárica normal o células de cáncer de ovario epiteliales. Se discute el uso de estos modelos para estudiar estromal-epitelial interacciones que se producen durante el desarrollo de cáncer de ovario.
Tags
Heterotypic Three-dimensional In Vitro ModelingStromal-epithelial InteractionsOvarian Cancer InitiationOvarian Cancer ProgressionEpithelial Ovarian CancersGynecological MalignancySurgical TreatmentsPlatinum-based ChemotherapiesEOC Survival RatesStage I TumorsStage III/IV DiseaseMortality RatesMolecular Genetic BasisBiological BasisEarly Stage Disease DevelopmentMicroenvironment RoleRisk Factors For EOCsNormal Ovary Co-cultureOvarian Surface Epithelial CellsStromal Fibroblast Cells
